2024 The single cell stereo-seq reveals region

The single cell stereo-seq reveals region

Author: yxbo

August undefined, 2024

WebAug 13, 2024 · Recent advances in single-cell RNA sequencing (scRNA-seq) and in particular single-nucleus RNA sequencing (snRNA-seq) have put the establishment of comprehensive, high-resolution reference transcriptome maps of mammalian cells and tissues on the agenda of international consortia such as the Human Cell Atlas (Regev et … WebMay 23, 2024 · The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves Keke Xia, Hai-Xi Sun, Jie Li, Jiming Li, ... Xun Xu Pages 1299-1310.e4 View PDF Article preview

The Single-cell Stereo-seq reveals region-specific cell subtypes …

WebApr 14, 2024 · 6.Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation. 单细胞转录组和翻译组双组学揭示了人类卵细胞成熟的潜在机制。 7.Structural basis for shape-selective recognition and aminoacylation of a D-armless human mitochondrial tRNA. WebDec 20, 2024 · The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves. Dev. Cell 57, 1299–1310 (2024). This paper reports the use of an in situ... a etica na escola

Dissecting the spatial distribution of specialized …

WebAug 16, 2024 · Single-cell sequencing workflow. Credit: Amanda Haage. The broad definition of single-cell sequencing is pretty self-explanatory: you take a single cell and … WebMar 31, 2024 · Stereo-seq experiments were performed as previously described (Chen et al., 2024). We used a Stereo-seq capture chip with an area of 200 mm 2 (20 mm × 10 mm). First, the chip was washed with NF-H 2 O supplemented with 0.05 U/µL RNase inhibitor (NEB, M0314L) and dried at room temperature. The embedded tissue was cut to a thickness of … WebApr 13, 2024 · We classified the Stereo-seq data into 6 clusters on the basis of their spatial information and histological features, including CIZ cells (cluster 0), inner cortex (cluster … kkr中目黒バス

The single-cell stereo-seq reveals region-specific cell …

Integrated single-nucleus and spatial transcriptomics captures ...

WebSep 6, 2024 · Single-cell Stereo-seq reveals new insights into axolotl brain regeneration Download PDF Copy Reviewed by Emily Henderson, B.Sc. Sep 6 2024 The axolotl Ambystoma mexicanum is a popular pet... WebSep 2, 2024 · Here we established the transcriptional landscape of cell types populating the developing heart by integrating single-cell RNA sequencing and spatial enhanced resolution omics-sequencing (Stereo-seq). kkrゆきぐに荘WebApr 10, 2024 · Single-cell RNA-sequencing (scRNA-Seq) is widely used to reveal the heterogeneity and dynamics of tissues, organisms, and complex diseases, but its analyses still suffer from multiple grand ... kkrメンバーズカード jcb

"WebOct 21, 2024 · Understanding the complex functions of plant leaves requires spatially resolved gene expression profiling with single-cell resolution. However, although in situ gene expression profiling technologies have been developed, this goal has not yet been achieved. Here, we present the first in situ single-cell transcriptome profiling in plant, scStereo-seq … " - The single cell stereo-seq reveals region

The single cell stereo-seq reveals region

Spatiotemporal transcriptomic atlas of mouse organogenesis using ... - Cell

WebMay 23, 2024 · Spatial enhanced resolution omics-sequencing—Stereo-seq—is a new technology that is based on DNA nanoball patterned arrays, which enable spatial analysis at an impressive resolution ( Figure 1 ). The diameter of one DNA nanoball is 220nm, such that there are 400 nanoballs in 100μm 2. WebSeurat is an R toolkit for single-cell genomics 10 where the SCTransform command replaces the NormalizeData, ScaleData, and FindVariableFeatures commands; this change helps identify rare cell populations by improving the signal-to-noise ratio. 11 Cells with ≤400 and ≥6000 genes and a mitochondrial gene percentage of ≥20% were filtered out ...

Did you know?

WebApr 13, 2024 · A single-cell Arabidopsis root atlas reveals developmental trajectories in wild-type and cell identity mutants. Dev. Cell 57, 543–560 (2024). Article CAS PubMed PubMed Central Google Scholar Xia, K. et al. The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves. Dev. WebUsing the Single-cell 3′ Library and Gel Bead Kit V3.1 (cat# 1000075 10x Genomics, Pleasanton, CA, USA) and Chromium Single Cell B Chip Kit (cat# 1000074, 10x Genomics), the cell suspension (300–600 living cells per µL determined by Count Star) was loaded onto the Chromium Single Cell controller (10x Genomics) to generate single-cell gel ...

WebOct 21, 2024 · Understanding the complex functions of plant leaves requires spatially resolved gene expression profiling with single-cell resolution. However, although in situ … WebOct 24, 2024 · Brain regeneration requires a precise coordination of complex responses in a time- and region-specific manner. Identifying key cell types and molecules that direct brain regeneration would provide potential targets for the advance of regenerative medicine. However, progress in the field has been hampered largely due to limited regeneration …

WebMay 23, 2024 · Xia et al. establish the single-cell spatial transcriptome technique (scStereo-seq) in Arabidopsis leaves and discover subtle but significant transcriptional differences … WebSingle-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration Xiaoyu Wei†, Sulei Fu†, Hanbo Li*†, Yang Liu†, Shuai Wang†, Weimin Feng†, Yunzhi Yang†, ... and region-specific manner. Identifying the cell types and molecules involved in this process would advance our understanding of brain re-

WebMay 1, 2024 · Here, we present scStereo-seq (single-cell spatial enhanced resolution omics sequencing) that enabled us to show the bona fide single-cell spatial transcriptome …

WebMay 4, 2024 · Although single-cell gene expression profiling technologies have been developed, their application in characterizing cell subtypes has not been achieved yet. … aetidia uspWebJan 27, 2024 · Title:The Single-cell Stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves,Data type:Raw sequence reads,Transcriptome or Gene expression. Home; ... Non-human Primate Single-cell Atlas; B10K: Bird 10,000 Genomes; FishT1K: Transcriptomes of 1,000 Fishes; Fish10K: The 10,000 Fish Genomes; … aeti global ltdWebwww.science.org aeti centrale a etica na religiaoWebJul 7, 2024 · In central infected zone of nodule, we found that uninfected cells specialize into functionally distinct sub-groups during nodule development and revealed a transitional subtype of infected cells with enriched nodulation-related genes. Overall, our results provide a single-cell perspective for understanding rhizobium-legume symbiosis. Figures Main kkr ゆづきWebMay 4, 2024 · Stereo-seq enables large field-of-view spatial transcriptomics at cellular resolution • Stereo-seq reveals the spatial cell-type heterogeneity of mouse embryonic tissues • Stereo-seq maps the spatiotemporal transcriptomic dynamics during mouse organogenesis • Stereo-seq defines the spatiotemporal window of developmental disease … kkrゆづきWebUsing the Single-cell 3′ Library and Gel Bead Kit V3.1 (cat# 1000075 10x Genomics, Pleasanton, CA, USA) and Chromium Single Cell B Chip Kit (cat# 1000074, 10x … kkr中目黒ホテルホームページ