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H9c2 2-1 cells

WebApr 4, 2024 · H9c2 cardiomyoblasts from rat embryonic cardiomyocytes were purchased from (ATCC, CRL-1446). Cells were cultured in complete DMEM (ATCC 30-2002), supplemented with 10% FBS (ATCC, 30-2024), and antibiotic penicillin/streptomycin (100 U/mL: 100 mg/mL) as described previously [ 14 ]. WebH9c2 cells were incubated with ET-1 in the presence or absence of KMUP-1 for 3 h. In order to measure ROS production induced by ET-1, cells were stained with 10 μM of DCFH-DA for 30 min at 37 °C, and immunofluorescence was visualized using a digital camera attached to an inverted microscope. Photographic images were taken of five random fields.

Klotho overexpression suppresses apoptosis by regulating the …

WebBased on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective … WebMar 22, 2024 · ( d) H9c2 cells were cultured in retinoic acid (RA) and 1% FBS for 5 days to induce differentiation and maturation. Differentiated H9c2 cells were then cultured with KL (200 ng/mL), FGF23 (100 ng/mL), KL (200 ng/mL) plus FGF23 (100 ng/mL), KL (200 ng/mL) + anti-FGF23 (1 μg/mL), or KL (200 ng/mL) plus anti-KL (1 μg/mL) for 3 days. reformat agency https://mcseventpro.com

The H9C2 cell line and primary neonatal cardiomyocyte cells show ...

WebSep 4, 2024 · What is the recommended seeding density for H9C2 cells (specifically in a plastic tc t25 flask)? We have been seeding at the recommended 10x10^4/cm^2 … WebJul 16, 2024 · Oxygen–glucose deprivation reduced the viability of H9C2 cells. H9C2 cells were deprived of oxygen and glucose for 6 h (incubated in glucose-free medium without fetal bovine serum, in a 1% O 2, 5% CO 2, 94% N 2, and 37 ℃ environments) and reoxygenated for 4 h (incubated in a high-glucose medium containing fetal bovine serum, in a 5% CO 2 ... WebH9C2 cells were lysed in ATP lysis buffer (S0026, Bain-marie Biotech, Shanghai, China). The insoluble material was precipitated by centrifugation at 4 °C, 12,000g for 2 min, … reformat abinitio

H9C2 (2-1) Cell Line from rat - Sigma-Aldrich

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H9c2 2-1 cells

Differentiation of H9c2 cardiomyoblasts: The role of adenylate

WebMar 31, 2024 · The exposure of H9c2 cell lines to HG resulted in time- and concentration-dependent decreases in HIF-1α and HIF-2α expression together with an increase in prolyl hydroxylase-1,2 (PHD1 and PHD2) expression, the main regulators of … WebCell information for H9c2(2-1). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.

H9c2 2-1 cells

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WebFeb 1, 2015 · 2.1. Cell cultures. H9c2 embryonic rat heart-derived (ventricular) cells (myoblasts) from ATCC were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum (FBS) under 95% air/5% CO 2, and subcultured when at 50–60% confluence. Notably, a higher degree of confluence was associated … WebApr 4, 2024 · NC inhibited the phosphorylation of the mammalian target of rapamycin complex 1 (mTORC1) cascade and proinflammatory proteins in H9c2 cells. Top: …

WebH9C2 (2-1) Cell Line from rat. BDIX heart myoblast, 88092904. View Price and Availability. Sigma-Aldrich. C-12375. Human Cardiac Fibroblasts (HCF) ... HPV-18, Hepatitis A, B, … WebWhen cultured in low-serum containing medium, H9c2 myoblasts exit the cell cycle and differentiate into myocytes/myotubes. A key step of this process is the expression of …

WebH9c2 (2-1) CRL-1446 ™. H9c2 (2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue that exhibits many of the properties of skeletal muscle. This cell line can be used in cardiovascular disease research. Product category. WebIsor hamnetin Reduces H2 O2－Induced Intrinsic Pathway of Apoptosis in H9c2 Cardiacmuscle Cells：The Role of ROS Scavenging and ERK／p53 Pathway. 2011-03 …

WebH9c2 cells (3.5 × 10 4 cells/cm 2) were treated with Era (1 μM), RSL3 (0.1 μM), or Fe-SP (0.5 μM) with or without GAA (2 μM) for 16 h. After these 16-h treatments, cells were …

WebCell line name: H9c2(2-1) Synonyms: H9c2 (2-1); H9c2; H9C2: Accession: CVCL_0286: Resource Identification Initiative: To cite this cell line use: H9c2(2-1) (RRID:CVCL_0286) … reformat according to styleWebOct 7, 2024 · Cells. H9c2 (2-1) rat-cardiac myoblast cells from ECACC were cultured on glass-bottom dishes (D11130H, Matsunami-Glass, Osaka) with DMEM including 1.8 mM CaCl 2 and 0.8 mM MgSO 4 (D6429; Sigma-Aldrich, St. Louis, MO) supplemented with 10% FBS, 100 U/mL penicillin G and 0.1 mg/mL streptomycin, in the 37 °C, 5% CO 2 … reformas tributarias 2023WebCells were grown at 1.2 × 10 5 in 6-well plates. H9C2 cells were cultured in high glucose (HG, 33 mM) or normal glucose (NG, 5 mM) during 21 days, changing the media each … reformat a locked usbWebH9C2 cells would continue to take in Ca2+, decreasing extra-mitochondrial Ca2+levels. Figure 2: The images on the left are the un-differentiated and differentiated H9C2 cells. After differentiation, the cardiac myoblasts turned into “cardiac myocyte-like” cells. Due to mutations that could have occurred, it is hard to tell their exact reformat alienware to factory settingsWebFeb 8, 2024 · The pcDNA3.1-MALAT1, MALAT1 siRNA (si-MALAT1) and their corresponding controls were purchased from Shanghai GenePharma Co., Ltd. H9C2 … reformat a hard diskWebJan 6, 2024 · Di bawah ini kami sertakan daftar produk cell line Elabscience. Jika Anda tidak menemukan cell line yang dibutuhkan, mohon untuk menghubungi: Whatsapp 081293185185. Catalogue No. Description. Size. EP-CL-0001. 293 [HEK-293] Cell Line. 1×10? cells/vial. reformata baptist church knoxville tnWebEfficiency of DNA transfection of rat heart myoblast cells H9c2(2-1) by either polyethyleneimine or electroporation. Expression of exogenous DNA in vitro is … reformat address in excel cells