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Graphene based gene transfection

WebThe use of NA is gaining increasing interest and more than 2000 clinical trials of NA-based therapies are underway. 2 The current advancements in medicine, biology and biotechnology have extended the scope of gene therapy from the “simple” transfection of plasmid DNA (pDNA) to the delivery of a variety of NA, including short interfering RNA ... WebMany researchers have tried to transfect genes using carbon-based materials such as graphene oxides (GOs) and graphene quantum dots (GQDs) [29,30,31,32]. ... Prior to in vitro gene transfection with NGQDs, we verified the cytotoxicity of the NGQDs via a CCK-8 assay kit. For the CCK-8 assay, various concentrations of NGQDs from 1 μg/mL to 1000 ...

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WebJul 1, 2024 · Fig. 1. Illustration of various carbon-based structures (graphite sheets, graphene, nanotubes, fullerene, and nanodiamond) that are frequently used in medicine and biomedical applications. 2. Gene therapy and carbon structures. Gene therapy simply means using therapeutic nucleic acids as drugs to treat diseases [7]. WebDec 1, 2024 · Graphene and its derivatives, as sheet-like carbonic nanomaterials, have been progressively utilized in various biomedical fields. Currently, applications of … city assessor of taguig https://mcseventpro.com

Targeted delivery of reduced graphene oxide nanosheets using ...

WebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex loaded with miR-7b overexpression plasmid. WebDec 1, 2013 · It can be summarized through these relevant studies that GO with suitable surface functionalization could serve as gene transfection vectors for in vitro and in vivo gene delivery. However, using graphene-based nanovectors as gene transfection vectors remains to be further explored. WebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … dicks sporting good first responder discount

Graphene and carbon nanotube nanocomposite for gene transfection

Category:Graphene based gene transfection - Nanoscale (RSC Publishing)

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Graphene based gene transfection

Graphene-based nanomaterials for drug delivery and

WebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … WebSep 15, 2012 · Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. …

Graphene based gene transfection

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WebNov 22, 2016 · Graphene based gene transfection. Nanoscale 2011; 3: 1252–1257. Article CAS Google Scholar Prencipe G, Tabakman SM, Welsher K, Liu Z, Goodwin AP, Zhang L et al. PEG branched polymer for ... WebWe conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency. ... Liu, Z. Graphene based gene transfection. Nanoscale 2011, 3, 1252–1257. [Google Scholar] Liu, Z.; Robinson, J.T.; Sun, X.; Dai ...

WebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also … WebApr 12, 2024 · Polymeric gene carriers target the nucleus and express their therapeusis through transcription and translation . Gene carriers are either viral vectors (retro-viruses and adenoviruses) or non-viral vectors. Viral vectors submit their genetic materials to the host cells and are known to attain greater transfection . 18.3.1.2 Polymeric Micelles

WebTo evaluate the transfection efficiency of NGQDs, we delivered mRNA into HeLa cells with NGQDs. As shown in Figure 4 a, the NGQDs formed complexes with mRNA at a 20:1 … WebMar 10, 2011 · Graphene is successfully used as a non-toxic nano-vehicle for efficient gene transfection and suggested to be a novel gene delivery nano-vector with low …

WebObjectives: This review describes the different dimensions of carbon-based nanocarriers including 0-D fullerene, 1-D Carbon Nanotubes (CNTs), and 2-D graphene and Graphene Oxide (GO) and their surface modification with different biocompatible and biodegradable molecules via covalent or non-covalent functionalization. dicks sporting good folding chair with canopyWebOur results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non-viral based … city assessor petersburg vaWebDec 7, 2024 · Graphene has been widely studied for biomedical use since its first appearance in 2004 [3], partly owing to its high Young's modulus, high fracture strength, high electrical conductivity, and excellent optical performance. Graphene also possesses a large specific surface area for adsorption of bioactive compounds and has high optical … dicks sporting good first colony mallWebMar 1, 2011 · Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for … dicks sporting good enfield ctWebOct 13, 2014 · Graphene based biosensors make use of two strategies-1) probe molecule on graphene interacts with the analyte (FRET based ... Zhang S, Liu Z (2011) Graphene based gene transfection. Nanoscale 3: 1252-1257. Zhou L, Wang W, Tang J, Zhou JH, Jiang HJ, et al. (2011) Graphene oxide noncovalent photosensitizer and its anticancer … city assessor polk county iaWebJan 27, 2011 · Degradable copolymer based on amphiphilic N-octyl-N-quatenary chitosan and low-molecular weight polyethylenimine for gene delivery. Liu C , Zhu Q , Wu W , Xu … city assessor sioux city iowaWebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of … city assessor\\u0027s