WebFeb 20, 2024 · The biotinylated RNA bound to proteins was pulled down from the cell lysate by streptavidin agarose beads. RNA was detected by RT-PCR (Figure 3) and associated proteins by mass spectrometry (Figure 4). Our findings show that an RNA aptamer tag to RNA molecule is an effective method to explore the functional roles of … WebFeb 25, 2024 · a, In vitro methods.Top, schematic of end-biotinylated-RNA pulldown. RNA is synthesized with biotin at the 5′ or 3′ end and …
Methods to study RNA–protein interactions Nature …
WebFeb 25, 2024 · a, In vitro methods.Top, schematic of end-biotinylated-RNA pulldown. RNA is synthesized with biotin at the 5′ or 3′ end and combined with streptavidin. Recombinant or cellular-extract proteins ... WebNov 5, 2024 · After extensive washing, the immobilized biotinylated RNA was eluted (for details see Material and methods: UV-cross-linking and affinity capture of protein–RNA … taiwan education system
Simple methods for the 3′ biotinylation of RNA - PMC
WebOct 30, 2015 · Anti-RNA blotting with chemiluminescent detection. 30 October 2015. This protocol is for northern blotting/dot-blotting to detect specific RNAs via chemiluminescence, using biotinylated DNA oligo probes. Gels etc are optimized for detection of 0.5-4kb mRNA transcripts. Much of it is adapted from ULTRAhyb-Oligo and Brightstar BioDetect ... WebThe biotinylated nucleotide is supplied as a pre-mixed NTP at an optimized ratio, providing convenient, consistent labeling. Highest amplification in a single round—up to 10,000 … WebNov 14, 2013 · A biotinylated uridine (U-biotin; Figure S1 in File S1) was then ligated onto the 3′ A-tail RNA by T4 RNA ligase. Excess U-biotin was then removed from the labelled RNA using a size exclusion spin column, leaving it ready for sensor surface immobilisation and subsequent SPR analysis with potential binding partner molecules. twin sayings tee shirts